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Plankton absorbs, Carbon-14 from the ocean much like terrestrial plants absorb Carbon-14 from the air.

Since plankton is the foundation of the marine food chain, Carbon-14 is spread throughout aquatic life.

When it comes to dating archaeological samples, several timescale problems arise.

For example, Christian time counts the birth of Christ as the beginning, AD 1 (Anno Domini); everything that occurred before Christ is counted backwards from AD as BC (Before Christ).

An extensive tree-ring sequence from the present to 6700 BC was developed in Arizona using California bristlecone pine (), some of which are 4900 years old, making them the oldest living things on earth.

Limitations and calibration: When Libby was first determining radiocarbon dates, he found that before 1000 BC his dates were earlier than calendar dates.

He had assumed that amounts of Carbon-14 in the atmosphere had remained constant through time.

In fact, levels of Carbon-14 have varied in the atmosphere through time.

One good example would be the elevated levels of Carbon-14 in our atmosphere since WWII as a result of atomic bombs testing.